TRAM: Translation Microscopy - From Confocal to Clarity
Affiliation:
IB3, EPS, Heriot-Watt University
Description:
Multi-colour TRAM-restored image of a pulmonary endothelial cell stained for actin (red), microtubules (green) and DNA (grey). The TRAM image was restored from 60 diffraction-limited images obtained using confocal laser-scanning microscopy.
Equipment used:
TRAM algorithm in MATLAB, SP5 gSTED SMD laser scanning confocal microscope (Leica) using a 60X 1.4 NA objective
TRAM: Translation Microscopy - From Confocal to Clarity
Full size image: TRAM: Translation Microscopy - From Confocal to Clarity